Thank you Mr Colomb for your kind reply. I went through your suggested article and it was very helpful. However I am interested in intermittent fasting. For that I am planning to give 1 hr, 6 hr and 12 hr starvation (on 4% sucrose solution) for 30 days. Also I am wondering what is best way to give starvation with- water, PBS or sucrose.

Rohit

In one type of our experiments, we starve the flies for 16 hours before starting the experimental measurements. We use just water on a chem wipe so they experience starvation without desiccation. If you give sucrose, that isn't really starvation. They eat the sucrose, and probably any yeast (or other microbes?) living on the sucrose.