While making two-photon microscopy for a mouse brain through cranial window (in vivo), how much power are you using when you are imaging at the deepest (e.g. 500 um, or even below)? How do you know what is too much? (We have Nikon A1R MP, with Ti:Saphire Chameleon from Coherent)
I found this article in JOVE (a video journal) which might give you an answer. This article is open access and you can watch the video and also download the PDF.
Marker, D. F., Tremblay , M., Lu, S., Majewska, A. K., Gelbard, H. A. A Thin-skull Window Technique for Chronic Two-photon In vivo Imaging of Murine Microglia in Models of Neuroinflammation. J. Vis. Exp. (43), e2059, doi:10.3791/2059 (2010).
The link to the article (video) is:
http://www.jove.com/video/2059/a-thin-skull-window-technique-for-chronic-two-photon-vivo-imaging
Happy Imaging!
Sathya Srinivasan
RUN Microscopy Core Facility Manager
University of Calgary
www.ucalgary.ca/runcore
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