How to plan a classical enzyme-substrate reaction ???

Hello everyone! I am mostly a structural biologist who did not get a chance yet to delve into a classical biochemical enzyme-substrate reaction. So as things would have it, I will have to perform...

15 October 2019 7,411 1 View

How to use C18 column to detect the presence of NADH/NAD+ in my protein and its mutants?

Dear Scientific community, I have a protein (tetramer) which has a NADH bound to it (confirmed from the crystal structure) when it is purified from Bacteria. Additionally, I have two mutants of...

13 January 2019 9,330 3 View

What is better to buy a real-time pcr machine or an endpoint?

Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...

24 January 2021 8,320 4 View

If we are going for co digestion of microalgae with wastewater but the sample is having low pH, What are the effective ways to increase the pH ?

wastewater with low pH

07 December 2020 2,716 3 View

Anyone working on Molecular Taxonomy?? I would be highly obliged if anyone could help me with primer selection and Phylogenetic analysis ??

I have a wide range of freshwater microalgae collected from various districts of Tamil Nadu. I am looking for a collaborator who could help me with molecular identification of some rare unknown...

23 September 2020 8,030 1 View

If the microalgae experience stress through transportation. What would be the best course of action to avoid it from becoming contaminated or killed?

I am currently doing an experiment in my faculty lab and I need living micro-algae to be transported from other countries. The samples will be done in Morocco and Spain and they will be sent to...

08 September 2020 4,424 2 View

How can I differentiate between cyanobacteria and microalgae in agar plates?

I'm trying to isolate cyanobacteria from fresh water and soil. I've plated several dilutions of my samples into BG11-agar plates and I have a mixture of green colonies/halos and brown stains which...

06 September 2020 8,959 4 View

How to determine the protein and carbohydrate of microalgae samples?

I used gravimetric analysis to determine microalgal lipid content after lipid extract in my lab. Now we need to determine both protein and carbohydrate content. We have a Velp TKN...

06 August 2020 8,166 4 View

Is it a good idea to build an reactor for Anaerobic Digestion or hydrogen fermentation using acrylic sheets or glass sheet?

Does using a reactor made of acrylic sheets beneficial for microalgae anaerobic digestion?

16 July 2020 9,062 2 View

Microalgae-biofertilizer-application methods?

02 June 2020 5,180 1 View

In Iran which field (macroalgae or microalgae) do you think is more important to spend and why?

Due to the lack of broad algae market in Iran and this image's information, do you think the activities in which the field (macroalgae or microalgae) is more necessary?

13 March 2020 4,887 3 View

What could be the reason for high activation energy during pyrolysis process?

I have a scenario where I used pelleted macroalage for pyrolysis. The heating rates were 20, 30 and 40 C/min. I had experience in using isoconversitional methods in the past were I have seen...

02 March 2020 3,217 5 View