How much is PEG size critical in Pichia LiCl transformation protocol? Is it OK to use PEG-4000 instead of PEG-3350?

More Tomáš Hluska's questions See All

Anybody with experience with whole plasmid sequencing? Should I worry about plasmid oligomers?

I've sent my plasmids for whole plasmid sequencing using the Oxford nanopore sequencing method. All of them were assembled as monomers, but in the reads (see attached pictures), there are even...

19 June 2024 1,280 5 View

What can be yellow colonies after E. coli TOP10 trnasformation?

Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...

16 June 2024 3,272 3 View

How to reduce the number of measurements/iterations needed in deep reinforcement learning?

I'm trying to use reinforcement learning with live EEG measurements. However, just 2000 measurements/iterations take 16.6 minutes to measure and it seems I need at least 10 hours of live...

12 June 2024 2,884 3 View

What can be wrong with this agarose gel?

A friend did PCR and then loaded it on agarose gel. All samples were prepared in the same way; the PCR was identical, with the same template, only primers differed, the same amount loaded on the...

04 June 2024 3,049 3 View

How can I see all points in a 5-95 percentile Boxplot in Graphpad?

Hi! I am trying to do some boxplots in graphpad. I would like to use the 5-95 percentile boxplot option, but if I choose this option I do not know how can I also see the individual values of each...

17 April 2024 6,093 0 View

Why could amplicon sizes appear bigger than they should on agaose gel electrophoresis?

I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...

09 April 2024 4,487 5 View

What kind of diatom is this?

I was looking at a sediment sample from a "biosphere" that I´m creating and I came accross this little guy. I´m almost certain that it´s a freshwater diatom but would like to know the specific...

19 March 2024 1,763 10 View

Does somebody have experience with ThermoFisher's Platinum SuperFi II DNA Polymerase?

I just found the Platinum SuperFi II DNA Polymerase, which should simplify PCR protocol as it allows uniform annealing temperature of 60°C, but it should also have very high fidelity; >300×...

17 March 2024 7,686 4 View

Me puede proporcionar iso 9001:2015?

quisiera que me proporciones un sistema de gestion de calidad

15 March 2024 3,491 1 View

Iso 9001:2015?

ME PUEDE PROPORCIONAR INFORMACION SOBRESistema de Gestión de Calidad Basado en la Norma ISO 9001:2015 para el Instituto de Educación Superior Tecnológico

15 March 2024 447 0 View

How to back transform the results generated from analyses using log transformed with In(X+1) data?

I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...

31 July 2024 7,860 3 View

In passive sampling, can polymer-lipid partition coefficients be applied independent of environmental salinity?

For example, if low-density polyethylene (LDPE) is equilibrated with PCB contaminated marine and freshwater sediments, can LDPE-lipid partition coefficients be used to obtain equilibrium...

28 July 2024 263 1 View

Can you recommend provider Intermediate laboratory test for hydrocarbon, polyethylene & polypropylene?

Accredited laboratory of JV "Uz-Kor Gas Chemical" LLC (Uzbekistan) is looking for providers to conduct interlaboratory comparison tests on gas chromatographic analysis of hydrocarbons (component...

25 July 2024 8,108 0 View

Swerling Characteristic functions?

Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...

23 July 2024 4,925 1 View

Radar Detection Probabilities?

Currently I need to calculate detection probabilities (PD) from RCS data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0 Equation. The idea is based on...

22 July 2024 2,851 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

Radar Detection Probabilities using beta distributed Scattering Cross section?

Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...

22 July 2024 868 0 View

VOC analysis on GCMS ?

Hi everyone, Can someone explain for me a method to inject VOC collected with Tedlar bag from pyrolysis process and transfer or inject into GCMS. Thanks

22 July 2024 6,398 1 View

Bimodal Distribution, Split or Transform for ANOVA?

Hi, I have some experiments where different nutrients/fertilisers (including but not limited to N) were applied to plants. Unsurprisingly the data produced is bimodal. The populations are...

18 July 2024 7,305 1 View

How can I quantify MDEA (N-Methyldiethanolamine) with a GC-FID?

I have a mixture of glycol, water, MDEA, and piperazine. I want to analyze it with GC-FID.

15 July 2024 9,652 3 View

Michael J. Benedik

They should be essentially identical for your purposes.

Dominique Liger

Hi there,

I agree with Michael, the slight increase in the average MW of PEG shouldn't make much of a difference... As long as it is a good quality PEG.

Tomáš Hluska

Great, thanks for your answers. Now I can sleep a little better :) And rest assure that the failure won't be because of PEG xD