How much dextrose should I use if I need to induce high-blood sugar in mice for a diabetes study? What dosage/concentration and kind are suggested? Also, should the mice be kept "plugged" into the dextrose source at all times?
Infusing dextrose would achieve a transient rise in blood glucose levels which is not synonymous wth induction of diabetes mellitus. Have you considered inducing diabetes with streptozocin or alloxan.
Hello, Sir. We proposed the use of alloxan and streptozocin to our adviser, but we were warned that this may take time. With this in mind, we looked for other possible options for inducing diabetes in mice. In relation to your suggestion, do you know any suppliers of alloxan and streptozocin that we can contact? Thank you!
Normal rodent islets have tremendous capacity to compensate for hyperglycemia when infused with glucose. Achieving a high blood glucose by infusing glucose solution, comparable to severe hyperglycemia caused by streptozotocin treatment for near complete destruction of beta cells, is almost impossible.
Ages ago, in case of normal rats (~150gm), I infused 50% glucose at a flow rate of 2ml/hr - within 2 days rats were able to compensate sufficiently that raising blood glucose over 200mg/dl wasn't easy and getting to >400mg/dl was impossible.
Both STZ and Alloxan can be purchased from Sigma Aldrich.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Metabolic Diseases
- Glucose Metabolism Disorders
- Diabetes