Probing pocket depth after periodontal surgery
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...
27 February 2021 5,681 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...
22 February 2021 1,400 4 View
Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...
22 February 2021 7,127 4 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View
I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...
16 February 2021 8,872 2 View
Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...
11 February 2021 3,292 3 View
I am trying to set up a new experiment where I am simultaneously utilizing reverse dialysis (antibody) through a microdialysis probe and a Miniscope for one-photon calcium imaging. Both pieces of...
09 February 2021 3,981 1 View
