How to calculate the ratio of PCR master mix and DNA?

Before running PCR what is the ratio of Taq, dNTP, Primers, buffer, water and DNA? is there any method for calculating?

17 December 2015 4,667 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

I've my Gold Nanorods in solution form and I have to conjugate them with drugs. Please go through description i have given?

I've my Gold Nanorods in solution form and I have to conjugate them with drugs. They remain in solution form after conjugation. Now for MTT assay for drug delivery on cancer cell lines, I am...

16 January 2021 3,992 1 View

Hello, can i learn amount of Chlorophyll in Spinach as a mg in 100 grams ?

I have looked on the internet and website but i could not find it so i need yours help please.

26 December 2020 8,354 3 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

I put a sodium chloride which is less than solubility chart but it doesn't melt. can you tell me what the problem is?

24 September 2020 224 3 View

How to coat seeds for lab experiments?

Hello everyone, We are planning to study how plant growth promoting bacteria are affecting various plants (eg. wheat, barley, etc.). We would like to coat the seeds with bacteria. We prefer film...

13 September 2020 8,721 7 View

How do I determine the amount of enzyme needed to liquefy a known quantity of starch substrate?

I have an enzyme preparation (powdery form) with an activity of 1045 U/g which I would like to use in mashing a 70 g of grain whose starch percentage is at 67%. How do I determine the quantity...

16 August 2020 1,998 5 View

How to find the densification of any electrolyte for solid oxide fuel cells/ How to calculate it ??

Most of the researchers used Archimedes principle to calculate its density. But that is not 100 % accurate. I want to calculate the density of pellet which is made by using 0.4-0.5 grams powder.

07 August 2020 1,049 4 View

Can anyone share with me their protocol for Picric acid for Pico Sirius Red?

1) How do I dissolve the Sirius Direct 80 Red? I know it should be in saturated Picric acid solution. But how many grams of picric acid should I add to dissolve in water? What should the...

22 March 2020 233 3 View

What are the research frontiers of Brewing Science? ....and how has brewery development impacted communities and Social interactions?

Microbrewing continues to expand across the globe with many US communities utilizing breweries as new social venues to meet-greet and participate in social activities. What do you perceive as...

19 February 2020 5,441 2 View