Are there any reagents that work well to directly conjugate fluorophores to antibodies of the IgM isotype?

We are developing a panel of antibodies for flow cytometry but several of the antibodies are of the IgM isotype. We are trying to find a kit or reagent to directly conjugate the antibodies to...

26 June 2018 465 2 View

My PCR is not working, and I have tried about any optimization. Any ideas?

30 August 2017 9,564 5 View

Sectioning whole rat brains (not blocked) in cryoprotectant solution for IF cFos?

29 November 2016 1,912 3 View

Does anyone have any experience with using TAT-Cre that they would like to share?

I am about to start some new experiments that rely on TAT-Cre (from Millipore) to delete a gene of interest in cell culture in order to study the impact on cell differentiation and gene...

04 October 2014 6,392 5 View

How can I learn more about your work on this project? Do you have anything written about it?

Hello! I'm do a lot of organizational work and much of it is focused on trying to create more space for inquiry as an approach to leadership (very much influenced by both narrative practices and...

01 January 1970 4,520 4 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View

How to properly calculate the fluid solid heat transfer between polypropylene matrix and glass fiber in a porous medium?

I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium

24 February 2021 1,915 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View