Hi! I am in the process of expanding HPMECST1.6R cell line. I already subcultured them twice but I need to expand them more. The problem is that due to power outage the humidified incubators are going to be out of power for two hours. I was wondering if the cells could survive and recover at room temperature and different CO2 conditions, inside the humidified incubator for a few hours?
I read that room temperature would not be an issue for 2 hours. CO2 could probably alter the ph, so I thought I could change the medium right after these two hours.