I'm trying to perform the isolation a compound from a reaction matrix, the stationary phase will be Silica gel.
Thanks,
Victor
There are many ways to do it. For the sample I prefer to put the silica directly on the sample (it is recommended to save a small sample for TLC), about the same weight as the sample, and then dry it with the rotavap until it is totaly dry, if it is necessary use some more silica and always meassure the quantities. For the stationary phase I use at least ten times the quantity I used for the sample preparation. For the column. You have to be sure that the column is clean and has no leaks. Then, I put a small piece of defatted cotton (use gloves) and a small quantity of anhydrous sodium sulfate. After that I put some dry silica gently (about the same quantity as the sodium sulfate). Then I make a mixture of the silica for the stationary phase with the weak solvent of the mobile phase trying to get rid of the air with the glass stirrer and then pour it gently inside the column. You should drain the mobile phase for the silica to compact and then add the sample slowly (leave a small quantity of liquid over the silica border), after that I put some more sodium sulfate and that's it. You can start the column chromatography. I hope my answer is useful.
You will need to be a bit more specific as their are many types and sizes of silica gel just as there are column sizes to pack them into. The particle size of the support, the internal diameter and length of the column, the liquid used to pack the column and the maximum pressure the column is expected to reach are a few examples of the basic information needed to propose a packing technique.
Once you provide the answers to those questions, you can use a key word search on the web to find examples of techniques used.
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