I want to preserve RNA extract for long time to use for qPCR and other experiment. How I can store it so it should not degrade with time?
Freezing in ethanol is the best way to preserve samples for long periods of time. Check out this bulletin for some details to consider, including RNases, storage and precipitations: https://www.thermofisher.com/jp/ja/home/references/ambion-tech-support/nuclease-enzymes/general-articles/working-with-rna.html
For the quick answer just search the section titled 'How to Store RNA'
Hi Shashi,
The easiest way to preserve high quality RNA extraction for long time is to freeze it directly at -80ºC. It works very well for us. But for prolonged periods, it's best to keep RNA at -80ºC but as a pellet under 96-99 % ethanol (use ethanol precipitation protocol to get the RNA pellet). Of course, as Abhijeet was suggesting, if the RNA will be used again for qPCR, you can simply make cDNA and store it at -20ºC. In this way, you'll store a more stable molecule.
Hope it helps,
Ruben
Shashi,
You can preserve by freezing at -80 degree in nuclease free water or mol.grade ethanol.
Transcription to cDNA is most adequate. Long term storage of RNA itself should be done at -80°C.
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