Dear Jehanzeb Ali Shah,
Please follow my advice, I hope it will be helpful.
Prepare two separate standard solutions of each. Take their zero spectra separately followed by taking 1st. Dv. of each. then check the two Dv.Sp. to find best wave length have sufficient distance from each other and with suitable sensitivity. Then make a suitable mixture of both and repeat the same procedure. Check if the two chosen wave lengths appeared separate enough, as excepted, that will be fine. If not and there was no other alternative, the consult, the paper of my M.Sc. Student here under the title:
"Four Spectrophotometric Methods ........etc. " under my name Fadhil M.Najib, and see if one of those four methods will be useful for you. Thank You and Good Luck.
For a 1st order derivative, the optimal wavelength would be the inflection point in a wavelength versus absorbance plot.
If you can export the data in Excel; the first column would be the wavelength (W), and the other column would be absorbance (A). The first derivative is estimated by (A2-A1)/(W2-W1), do that for all the cells in Excel. Plot the first derivative vs. wavelength. Hope that helps.
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