Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

Running lammps lmp_mpi (commande introuvable)?

20 March 2020 9,773 2 View

Are there examples of a highly impactful paper being published in a very low impact journal?

Are there examples of a highly impactful paper being published in a very low impact journal?

23 October 2019 1,918 6 View

Looking for a test data set in the Gynecology field?

I am looking for data sets containing real-world data for gynecology field. I would be grateful for suggestions of where I might find such data sets.

08 January 2019 1,533 1 View

Ask for explanation of a transient profile file of a bounary condition?

Hi all, The inlet boundary condition in my case is "mass flow rate". In this boundary; the temperature change with the time. I created a profile file (.prof) which contains the value of the...

12 August 2016 5,174 2 View

How can I over express an active protease in E.coli BL21 (DE3)?

31 March 2016 5,570 5 View

Is anyone familiar with the reinforcement of a steel beam ?

If a beam is damaged, corroded or cracked, how could be reinforced ?

27 January 2015 5,878 9 View

How can I measure a reliability index for civil engineering structures?

07 January 2015 7,642 11 View

Lammps: define interatomic potential 'problem'

hello I'm running into problems with my LAMMPS simulation. I created an in put file i, and ii define potential eam style): pair_style eam/alloy pair coeff * * Agu3.eam. Ag .Ag i have error:...

01 January 1970 2,228 4 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View

How to overcome convergence issues in concrete arch models?

Hi, I have modelled a three-span arch bridge with soil fill over along with pedestals. The FE modelling is done in LUSAS with HX8M solid elements (i.e. 3D solid modelling). The non-linearity is...

20 February 2021 1,346 2 View

How do I connect DAB DC DC Converter to a battery in matlab simulation ?

I am trying to model a DAB converter and I need to observe the power transfeered to the load side on varying the phase shift between two bridges, hence I couldnt take a constant load e.g...

09 February 2021 6,489 3 View

What happens in the salt bridge of Hg/HgO electrode?

My Hg/HgO electrode was used for EIS test in 4M NaOH solution. I found something in the salt bridge. After that, my EIS results found the noise at a low frequency (tested in a faraday cage)....

16 January 2021 1,900 1 View

Does anyone know about vision-guided control of civil structures?

I would like to know more about the following issues: 1. Using images as feedback for the closed-loop control of civil structures 2. Simulating the vision-guided control on the computer without...

03 January 2021 7,566 1 View

I have a challenge with a structure I optimized it's weight but the frequency constraint is not satisfied?

I know that frequency depends on material properties, geometry, support conditions and in plane loading. I have played around with these figures except that I maintained the same material steel...

26 December 2020 4,877 9 View

Why is shell element preferred over solid element in ANSYS APDL for bridge modeling in finite elements?

For modeling bridge deck slab on ANSYS APDL, why is shell element preferred over solid element? I read somewhere that the mesh of shell elements is more efficient than solid elements. Why is that?

02 December 2020 8,222 3 View

How to reduce (which reducing agent) a disulfide bridge in a protein and how long does it remain reduced?

I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...

24 November 2020 3,211 5 View

What is the type of connection between elements on ANSYS APDL after I merge their nodes together?

For the bridge shown below, the web is connected to both flanges, and the slab is connected to the parapet by meshing and merging the nodes, but what is the name of this connection? is it...

22 November 2020 2,288 5 View

How can I get the axial stress for an inclined beam188 element on ANSYS APDL?

I modeled a bridge on ANSYS APDL and added cross bracings / diaphragms as shown below. The diaphragms are not straight along the x or y or z direction, they are inclides BEAM188 elements. how can...

14 November 2020 8,172 3 View