How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What are the procedures for environment friendly and effective methods to prepare silicon QDs, Si nanotubes and silicene (2D materials)?

There are different nanostructures of silicon like nanotubes, QDs and silicene. I need to know what are the 'green' or other effective methods for preparing them..please share the procedures of...

10 January 2021 3,418 1 View

Is surface embrittlement unavoidable after spray coating with brittle powder ?

I am not an expert at materials science. However, when I look at the literature, I read a process called cold spraying. This process is basically spraying a ductile powder (e.g. copper) onto a...

07 December 2020 5,378 2 View

How to prepare lignin basic media ?

I want to prepare bacterial media containing Kraft lignin ( dissolved in 0.1 M NaOH). after I autoclave,the lignin gets precipitated. Please suggest how to retain the solubility of lignin in the...

07 December 2020 800 3 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View

How is the adhesion behaviour of spray coatings with subsequent sintering process?

How do ceramic coatings adher to the base substrate and what happens in the small diffusion zone? How are the binding properties? (Especially between TaC coatings and Graphite as base...

18 November 2020 2,930 4 View

How to amplify lignin degrading gene?

Hello colleagues, I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are...

20 October 2020 9,654 3 View

What is the possibility of side reactions during atmospheric plasma coating of NiCrBSi with Graphene?

I want to coat NiCrBSi metal matrix composite with Graphene Nano Platelets as reinforcing agent. The coating is to be developed by atmospheric plasma spraying method. I am in doubt on possible...

05 October 2020 5,102 3 View

What is the best procedure to extract bast fibre from plant bark having higher lignin content?

I have tried to extract fiber from the plant bark having higher lignin content with all traditional fiber extraction methods as well as treated up to 20% NaOH Solution at 100 Degree C. However...

04 October 2020 8,149 1 View

How to recognize and verify the crystallization occur or not?

Hi, everyone My feedstock powder is water atomized Sn with a diameter range from 10-100 um. After the cold spray processing, the EBSD maps can be obtained. Because my smallest powder is 10um,...

07 September 2020 7,766 4 View

How to prevent leaks in Swagelok fittings?

I am carrying out a liquefaction reaction inside a 2.5mL volume reactor made of Swagelok fittings (one SS 3/4" STRAIGHT UNION with two SS 3/4" PLUGS FOR FITTING). After adding the substrate...

11 August 2020 4,211 2 View