We carried out the electronic absorption measurements in DMF and we get a number of small sharp edges in both ligand as well as its complexes with Co Ni Cu Zn in the range of 200-260 nm.
Can you better define what you mean by "small sharp edges"? Even better, can you post the spectrum. At first guess, DMF absorbs in that region and it sounds as though you are just seeing jagged noise there as the spectrometer tries to subtract out the solvent blank.
Brajendra Kusmariya..I do a little uv visible spectra and fluorescence, these type of sharp edges I encounter when
1) Sample cell may need to be cleaned properly
2) Careful sample preparation
the region you discuused is the one absorbed by solvents. The spikes may be due to several causes such as problem is internal caliberation of your device, Improper functioning of the lamp. There is a little chance that these may be due to your sample
As I suspected, those features are just noise. When you subtract out the absorption of the solvent, there is very little left. I don't know if you are using a double-beam instrument or one where you electronically subtract the solvent baseline, but in either case these are not features, just noise.
Yes it affects, because of DMF is transparent from 275 nm with increasing function T[%] = f(lmax) to 320-350 nm. You can check the transmission "window" for spectroscopic solvents in [Ref. 1].
[Ref. 1]. UVASOLE: Loesungsmittel und Substanzen fuer die Spekroskopie, Reagenzien Merck; E. Merck, Darmstadt.