How to show a protein is being dimerized in SDS-PAGE gel?

A protein dimerizes through CTD. I have expressed CTD of protein with two different tags. Now, I want to show that these CTD of protein dimerize in a pull-down assay. But my doubt is that if the...

29 January 2021 7,529 4 View

What are the impacts of pKa1and pKa2 in drug absorption ?

Pregabalin is a crystalline solid with a pKa1 of 4.2 and a pKa2 of 10.6. what insight can i get from this aforementioned information during drug design. What is the difference between pKa1and pKa2.

28 December 2020 7,237 3 View

How to deal with negative factor loadings while performing PCA-MLR to calculate quantitative source contribution in air pollution studies ?

If I am getting some negative values in factor loadings while performing principal component analysis (PCA), then I should use that negative values to calculate daily source contribution using...

22 December 2020 5,248 1 View

What are the source markers of Naphthalene, Acenaphthylene and Acenaphthene in ambient atmosphere ?

Is any article available which reports the sources of these PAHs ?

22 December 2020 8,738 1 View

Why high infiltration in OM treated plot?

We are carrying out trial in the glasshouse where we are comparing the infiltration rate in sodic soil in two different treatments Polyacrylamide vs organic matter ( lucerne pellet). within 27...

25 November 2020 8,787 3 View

Where can one download data (csv, excel) for average daily values of AQI (air quality index) in popular cities, like, Delhi and Mumbai in India?

Air pollution has become a very big problem in Indian cities, especially Delhi. It requires immediate attention and we need to soon understand the trends, provide forecasts for future, issue...

19 November 2020 1,433 3 View

What is the best feasible method to plot DISTANCE vs LST graph?

The above question is intended to be answered in reference to the reduction of the urban heat island by the river.

22 October 2020 7,732 4 View

Which type of measuring instrument can be used to measure hot thermic fluid flow rate?

The flow rate of thermic fluid is less than 1 L/min and temperature ranges from 25 - 200 degree Celsius with a pressure limit of 5 bar.

06 October 2020 5,612 11 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

How to calculate a flow rate over the broad-crest wear for this conditions?

How to find a relationship between velocity and weir landing length (X') as shown in figure?

28 September 2020 7,159 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

No title

03 March 2021 5,360 2 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View