Cannabis Oil Winterization at -80oC

Hi everyone, I am currently running CO2 extractions and winterizing the crude material with 200 proof ethanol at -20oC for 12-24 hours. I am interested in speeding this process up by performing...

05 June 2017 7,900 18 View

How do you calculate the average pair-wise variation V to determine the optimal number of reference genes using GeNorm?

I am using the Bio-Rad CFX Manager Gene Study tool as my GeNorm interface, but I am not sure how to determine the optimal number of reference genes to use by calculating the V value for V2/3,...

07 August 2014 1,429 5 View

Does anyone know how to determine the significance of differential gene expression using RT-qPCR data?

I have RT-qPCR data obtained from an experiment where I am looking at gene expression of individual ORFs within an operon in response to a particular treatment. I have shown that Gene 1 is...

05 June 2014 9,597 20 View

Can you troubleshoot my problem with Norgen Biotek Total RNA Purification Kit?

I am using the Norgen Biotek Total RNA Purification Kit for isolation of RNA for RT-qPCR analysis. The RNA obtained is high-quality and in good quantity, but I have been having problems with...

05 June 2014 338 6 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View