I have an experiment consisting of many rounds divided into two stages and it's based on a between design. For each stage there are multiple rounds and due to learning effect subject behaviour is bound to exhibit a time trend. I guess I can use average for mean when I calculate the means, but what do I use for standard deviation when computing the effect size (eventually what I'm looking for is what is the ideal sample size)? Will greatly appreciate it if anyone can answer this question. Thanks in advance.