How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

Why would looking at the expression of mecp2 in the prefrontal cortex in rett syndrome be useful?

I don't believe there's much research in this area in humans. There are mice and primate studies

05 December 2020 4,556 2 View

Which simple task most reliably activates prefrontal cortex?

For equipment testing purposes my research group wants to acquire an fMRI data set with frontal cortex activation, as anterior as possible. What would be the most reliable (yet simple) task to...

24 November 2020 1,132 1 View

Label Propagation - Multi Atlas based 3D Image segmentation implementation. The basics?

How do i get started with the implementation of Label propagation (Multi Atlas based Image segmentation) using sparse coding? The same has been implemented in the paper Representation Learning: A...

17 November 2020 8,569 7 View

Anyone familiar with antioxidant studies on rats' brain homogenate?

I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...

04 November 2020 5,481 1 View

Where can I find coordinates of specific regions of interest (ROI) in the human brain?

Hi All, I need to specify a few Regions of Interest (ROI) in the human brain providing their coordinates (x,y,z). What I am interested in at this point are anterior cingulate cortex, dorsolateral...

01 November 2020 8,442 7 View

How does mind calibrate our eyesight and how can we use it as an application?

First, I have a theory in mind: Imagine the wiring in our minds that connects the neurons to our visual cortex. In the image sensors, we have a defined array of sensors. Hence, we can directly...

26 October 2020 1,230 4 View

Effect on MARK4 activity and microtubule of alzheimer mice brain?

I have to determine the effect of some phytoconstituent on MARK4 and microtubule of neuron. How can I do it. I mean do I have to first extract the MARK4 from hippocampus and cortex by RT PCR and...

12 October 2020 653 1 View

Do you think that proinflammatory cytokines such as TNF-alpha, IL-1beta and IL-6 may act differently in the prefrontal cortex and hippocampus?

Hello; In terms of learning and memory, do you think that proinflammatory cytokines such as TNF-alpha, IL-1beta, and IL-6 may act differently in the prefrontal cortex and hippocampus? I've some...

08 September 2020 7,032 3 View

Hello everyone, can you point me to a good antibody to label PV inhibitory neurons in the cortex at P7?

Hello everyone! I need to label mouse PV inhibitory neurons in the medial prefrontal cortex at an early post-natal time point (P7). However, I am experiencing some problems finding a good antibody...

08 September 2020 4,549 1 View