Hi Sruthi,

you should always have a marker on you gel - what you don't have. This is really suboptimal for analysis.

However, I think that it is likely that you have the 28s and the 18s band but you have also a strong signal from smearing low in your gel which might indicate at least some RNA degradation which could influence subsequent application negativily.

You could try it with this RNA if you don't get better RNA but I would advice to don a new RNA preparation to be on the save site.

I hope this helps.

Thanku mam..

Yes, you have got it right Sruthi. These two bands are of 28S and 18S rRNA.

Thanku for the conformation mam