How do you perform the hygiene of the outpatient material (ear speculum, tweezers, spatulas, nose speculum, nasofibroscope)?

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Medium replacement interfering with cell culture blot results?

We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....

17 June 2024 1,649 0 View

Z-axis alignment in a protein for z-axis constraints in Amber software?

Good morning, I am running some Steered molecular dynamics in Amber 22 and want to set some restraints in the z-axis so as to imply certain distances from my ligand to its substrate. My goal is...

27 May 2024 7,082 1 View

Analytical frequency calculation error in ORCA 5.0.3, does anyone know how to change the calculation algorithm?

Good afternoon, We have recently started running ORCA calculation in a new machine, however we are consistently getting memory errors in analytical frequency calculations. Our jobs com-prise a...

27 March 2024 8,609 2 View

LiF impurity on XRD data after HCl/LiF etching of Ti3C2 MXene ?

Hi, I've been etching Ti3AlC2 MAX phase in the lab for a year, having made several batches. In the last 3 I've been observing a peak at around 44°. This peak is observed for multilayer and...

11 March 2024 5,920 0 View

Can a medium containing 0.5% of FBS induce instant and drastic morphological changes in culture cells?

I'm doing a protocol in which I plate my cells in a 12-well plate with complete medium (i.e. DMEM/F-12 WITH 5% FBS), let them reach ~80% confluency and then I change the complete medium for a...

10 January 2024 8,232 5 View

How do I search a name in Zoological Record today?

Zoological Record today belongs to Clarivate and at their website, when I click at "Go to product" I am directed to Web of Science basic search. I did not find any filter for Zoological Record...

03 November 2023 8,752 1 View

How can I correctly interpret Tafel slopes?

Dear colleagues, I am having some doubts about how to correctly discuss the Tafel slopes. By definition, they show how much it would be necessary to increase the overpotential to increase the...

18 September 2023 4,959 3 View

Can I remove WAVECAR file after calculation in VASP?

First, I made a relaxation calculation of my systems. After this, I made charge calculation to export the CHGCAR and I used it to calculate de DOS. After DOS calculation, I removed the CHGCAR....

28 July 2023 2,580 2 View

E. coli electroporation transformation: Longer Recovery Time?

Hello! When trying to transform E. coli DH10B or TOP10 with a large plasmid, like more than 65k bp, what do you think is the best time for the cells to be let recovering during the recovery time...

31 March 2023 8,887 2 View

Fatal error: Group Water_and_ions referenced in the .mdp file was not found in the index file. Help me?

Hello ,guys! I'm trying to balance my system with NVT, but the following error appears: Fatal error: Group Water_and_ions referenced in the .mdp file was not found in the index file. Group names...

17 November 2022 6,479 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Why is trifluoroacetic acid (TFA) used in c-18 column?

I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.

23 July 2024 8,075 3 View

Can we add calcium carbonate to any media as an indicator for lactic acid producing bacteria?

As mentioned in literature the addition of CaCO3 to MRS Agar media is considered as an indicator for lactic acid producing bacteria, so I wonder if we can add CaCO3 calcium carbonate to any other...

20 July 2024 7,504 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

Should all lactic acid bacteria be catalase negative? should all probiotic bacteria be catalase negative?

I am working on probiotic bacteria for fish, and I am trying to isolate probiotic bacteria like lactic acid bacteria or other probiotics from the gastrointestinal tract of fish using different...

12 July 2024 3,756 4 View