How do you identify a gene from a whole genome of a species when the gene is not sequenced in the species?

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Vinod Kumar Jatav

dear sir

you can use BLASTX

WITH GENOME ALIGNMENT OF KNOWN SPECIES GENE LIKE TYPE- AND TYPE-2 AND YOU COMPARE THIS TYPE2 TO YOUR TYPE-1 GENOME

Jie Li

You should tell us where you got the sequence of this gene. Is it from a related close specie or you clone it from this organism?

Surajit Debnath

The whole genome sequence is available in GenBank but a common gene is not sequenced or specified for this species. The sequence is hidden in the whole genome sequence. But for other related species the gene is specified as cDNA / mRNA / peptide. I want to make primers for the gene of the species. Plz suggest. i have BLASTed related species sequence cDNA with the whole genome , but not of much use.

I think you should find the conserved sequence for this gene in related species which means it is the most possible similar sequence in the organism you need. Usually using coding region rather than introns or else.