How do scientists choose the most suitable restriction enzyme for cloning a specific gene?

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I want to understand what criteria scientists use, such as recognition sites compatibility with vectors, and the size of DNA fragments. Curious about the role of factors like sticky ends vs blunt ends.

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What are the current challenges and future prospects of integrating artificial intelligence into recognition systems for autonomous vehicles?

This question aims to explore the intersection of artificial intelligence and autonomous vehicle technology. It seeks to identify the key challenges faced in implementing AI for recognition...

20 July 2024 3,469 2 View

Help me download paper?

I have 2 papers below, but I can't access this, you can help me? Shuai Zhang, Xiaodi Li, Xingyu Zhou, Yuning Wang, Yue Hu, Cloud removal using SAR and optical images via attention mechanism-based...

18 July 2024 9,635 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

Hello friends In simulating the drug with the liposome, which side of the liposome membrane is the drug placed on? The top leaf or the other?

Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...

29 June 2024 9,688 1 View

What is the difference between opportunity recognition in entrepreneurship literature and sensing in dynamic capabilities theory?

While I do have some opinions on how to address this question based on my reading as student, I would like to know the opinions of more accomplished scholars.

28 June 2024 802 3 View

Can I premix the reagent for restriction digestion and make a mastermix like PCR?

Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...

24 June 2024 1,171 3 View

I am working on a network for facial expretion recognition and I have problem with the loss function can anyone help?

I am using dice loss and wing loss for loss function and my network outputs are heatmaps and landmarks and I am trying to train on both of them at a same time do you guys know how to solve this...

22 June 2024 10,013 2 View

Is the pure phonemic content related to emotional valence?

Dear colleagues, After statistical processing of a large corpus of English utterances assessed for emotional valence, it turned out that the phonemic content of speech is tied to emotional...

17 June 2024 5,459 0 View

Is it really worthy to have "Recognition Certificate" from unknown and unverified source?

Why might obtaining a recognition certificate from an unverified, small group NGO or a predatory award (such as the "Best Young Scientist Research Award") be considered less valuable or even...

12 June 2024 1,616 2 View

Will this 3C Analysis work or are the fragments too small/close together?

I'm doing a 3C analysis shown in the picture linked and attached below with the Bait fragments labeled and the target fragments that will be tested for interaction with the bait labeled with...

02 June 2024 8,038 1 View