CODE SNIPPET :

Assuming : Root is the name of the folder within which you have 40 subfolders each with say 10 images.

%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%

Path=' Root';

FolderR=dir(Path);

[Sz,xx]=size(FolderR);

for i=3:Sz %%% 1 and 2 are current and parent directory

SubN=FolderR(i).name;

SubP=dir(SubN);

[Sz2,yy]=size(SubP);

for j=3:Sz2

FileN=SubP(j).name; %%%Or to open

Path2=strcat(SubP(j).folder,'\',FileN);

Img=imread(Path2);

%% You need save or process here else it overlaps%%%%%

end

end

Thanks, Aparna. I appreciate

Thanks, Aparna