Having trouble with qPCR amplification plots. My curve's never seem to reach a plateau. Any ideas?

The parameters of the curve all seem acceptable, typically reactions results are above 0.9852, -3.1 to -3.6 slope, and 90-110% reaction efficiency. The results seem to be what I would expect from...

04 February 2021 1,138 3 View

QPCR standard curve troubleshooting advice?

Hi all, I am trying to set up my standard curve for qPCR analysis, presence absence of D. septosporum, using a QuantStudio 5 real-time PCR system and associated software. I am using a 5X...

25 November 2020 710 3 View

Can anyone help with setting up the R germinationmetrics Package using multiple species and treatments?

I have 14 species, 6 temperature settings and two treatments to examine germination responsiveness over 59 days. I want to start with some basic info for plotting (t50, Gmax etc). I want to...

18 November 2020 7,160 4 View

How to extract patient specific 3D geometry from GE Vivid E95 3D/4D echo?

I am needing to obtain patient specific geometries of the left ventricle for use in CFD and 3D printing. Does anyone know how to do this using 3D/4D echo obtained from the GE Vivid E95? Does this...

29 September 2020 2,614 3 View

Dear Researchers, what are the best input variables that should be selected for artificial intelligence in prediction of rock failure precursor?

Using of artificial intelligence modeling techniques for prediction of rock failure precursor

19 September 2020 1,981 2 View

Ideas for knocking down protein that is heavily upregulated in cancer?

Hello all, I am trying to knock down a protein that has been documented to be heavily upregulated in oral squamous cell carcinoma (confirmed via western blot). I have attempted to knock down this...

31 August 2020 9,214 3 View

How to exchange the buffer of peptide ~1KD?

This is a peptide about 1KD in size. The commercially synthesized powder was reconstituted with a citrate buffer(pH 7) and the solution turns acidic, pH ~4. The assay requires a pH at 7 so some...

31 August 2020 870 6 View

Error when running both DEM and Bio file in Maxent?

Hello everyone, I am Trang. I have problem when used the Maxent to predict the potential distribution of some rare speices in Vietnam. I have 2 file *.ascii, including DEM and bio9. Althought all...

20 July 2020 8,440 4 View

How to calculate error in photocatalytic data?

Hello, I made some measurements using a UV-vis spectrometer at intervals of 5 minutes to see the degradation of a resazurin dye by TiO2 under UV light. I did not repeat these measurements. How can...

23 May 2020 1,373 3 View

What is the ideal retention interval for measuring retention of knowledge?

If you're using an intervention in classrooms and testing knowledge at pre-intervention, post-intervention (immediately after) and then again at a retention time period, how long is the ideal...

19 May 2020 307 8 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View