How do you annotate figures to show significance?

30 January 2021 1,008 3 View

How can peptides based on allele sequences be delivered to mice?

I am looking to measure allele-specific immune responses for specific antigens. After identifying the most prominent alleles in a population, I am looking to express them in mice and then measure...

03 September 2020 9,281 1 View

Missing values in R lme(): how to include observations with missings in the outcome variable?

Hello, I am struggling to understand how I best use the na.action options in R's lme function for a hierarchical linear model. I have a longitudinal data set with a two-level nested structure...

23 July 2020 2,327 4 View

Warning messages in Abaqus relating to general contact, tie constraints, and stringer beam elements - are they an issue?

Hi, I have a 3D model that I am using to model a shear connections in reinforced concrete. The model is using C3D8R elements for the concrete and B31 elements for the reinforcement, which are...

27 February 2018 8,169 3 View

Liquid culture colony PCR technique?

I need to do a pcr on a liquid culture of E.coli. I'm amplifying a 150bp region of gyrA, using primers that I designed. I'm using NEB Next Ultra 2 Q5 MM. For the reaction, I am using 5ul NEB, 0.5...

25 October 2017 5,995 5 View

I am currently dealing with article critique on learning theories.? This been my first time to do this what would you recommend for effectiveness?

I am taking Edu 831 (Learning theories), it involves article critique on use of different learning theories in research. How can I critique an article effectively and what does it involve?

08 October 2017 1,958 3 View

How can research on attitude change benefit government initiatives to encourage young people to develop more healthy attitudes towards alcohol?

How do you think research on the Theory of Reasoned Action (TRA) and the Theory of Planned Behaviour (TPB) influence government initiatives to encourage healthy attitudes and perhaps even change...

12 August 2017 2,479 3 View

I would like to store 10 uL aliquots of bacterial culture. What would be the best way?

I need to store 10ul aliquots of bacterial culture , would the best way be to put them in PCR tubes with some gylcerol (10ul 50%) at -80?

31 January 2017 2,848 3 View

Unusual pelleting with ethanol precip after phenol chloroform. Help troubleshoot?

I've been using phenol chloroform extractions on my Chromatin IPs, but after the ethanol precipitation I either get very tiny pellets, or a smear along the side of the tube. I think it is...

07 June 2016 10,024 2 View

How do you compare splines fitted to repeated measures within & between groups?

I have repeated measures of a serum biomarker in 60 patients. 30 patients had pneumonia and 30 had asthma. Measurements were taken whenever blood was drawn during routine care, not at specific...

24 April 2013 3,724 6 View

How to manually calculate p value in ANOVA? what is the equation or formula for it?

How to manually calculate p value in ANOVA? what is the equation or formula for it?

03 March 2021 4,928 5 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

Bayesian decision trees and bayesian regression trees in Python and/or R?

I am looking for implementation of bayesian decision trees and bayesian regression trees in Python and/or R.. Do you know if the existing libraries support this

23 February 2021 6,689 3 View

How to assure whether a regression tree overfit or not by seeing bias-variance value of the model?

Lets consider a regression tree in which variance is 1.1065*e-10 and bias is 2.962e-13. Also the model RMSE on training set is 1.5e-5 and on training set is 1.2950e-5. Is the model is overfit or...

22 February 2021 7,755 2 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

How to properly reach normality in residuals with lmer?

Hello, I have a large non-balanced panel dataset regarding a company dealing with private lessons for students. My goal is to fit a lmer model which predicts the number of monthly hours taken by a...

08 February 2021 2,539 7 View