I am currently using PerkinElmer Uv-vis system. For the reflection correction the manual says to do both the Light spectral reference (LSR) and dark spectral reference (DSR) corrections. It also says that LSR is supposed to be provided by the instrument supplier that I dont have. Is it possible to make my own with white spectralon and then save it as .asc file to use later? Thanks in advance.
If the device does not provide the reflectance spectrum, you can measure the transmission of the reference sample (the cell with solvent only) taking as reference no sample (air). The subtraction of the two spectra is a measure of the reflection spectra of the reference sample. Most of the devices provide the reflection spectrum. You go to the setting of the program to find this feature. They also automatically correct for reflection losses.
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