Research minor requires us to determine the effectiveness of a cleaning product based on the atomiziation of Bacillus strains to hopefully cause competitive exclusion.

The full procedure consists of atomizing peroxides, washing that off with sterile water, then atomizing a cleaning product consisting of deodorizing agents, B. subtilis, amiloliquefaciens, pumilus, licheniformis and megaterium and four unknown Bacilli.

We take swabs before the cleaning procedure to use as reference, then two office wings will be cleaned, one with chemical products, the other with the product based on bacteria.

Does anyone know of a way to differentiate between commensal bacteria present before the cleaning of the surface and the ones that will grow after the atomizing of the product?

Assuming Bacilli are present before the cleaning as well.