I autoclaved a culture medium (LW) to which I have to add a solution of biphenyl. I prepared this solution in 100% alcohol. My problem is that when I add biphenyl to the cooled medium, it flocs immediately and does not get homogenized. I tried to add the solution in a little of Nutrient Agar but there is no real dissolution. How to proceed?
Preparation of Medium: Add components, except biphenyl solution, to 1.0L distilled/deionized water. Adjust pH to 6.9. Heat and gently bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Add an aliquot of the biphenyl solution to the lid of a sterile Petri
dish so that the final concentration will be approximately 0.25g/L biphenyl,
and let the ethanol evaporate so that the crystals of biphenylcoat the lid of the Petri dish. Aseptically add sterile agar medium to the
crystal-layered Petri dish.
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