How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

How the self defense mechanism of plants (either morphological or physiological) be used as positive parameter for crop protection ?

the defense mechanism of plant is really a wondrous mechanism if it can be utilize it will best way to deal with management practice for crop protection.

07 December 2020 780 10 View

Which problems we can find with the acquisition of a foreign language?

It is known that Spain shows a low level of use of English. But why?

01 December 2020 1,177 4 View

What solvents can be used to dilute the Lepidopteran defence droplets (scoli glandular secretions from lepidopteran larvae)?

I am trying to analyse the chemical profile of defensive droplets from the lepidopteran larvae (T Trypanophora semihyalina ). when i collected and tried to dilute in the hexane and acetone the...

23 September 2020 2,994 3 View

Can induce resistance may serve as a source to divert plant photosynthates to defense pathways?

Systemic acquired resistance (SAR) and induced systemic resistance (ISR) are two forms of induced resistance wherein plant defenses are preconditioned by prior infection or treatment that results...

21 September 2020 7,491 3 View

Can the math teacher in the 21st century work without technology with the Covid-19s preparing to mutate?

In a teaching world, which is becoming unpredictable right now, how can we use technology without losing our teaching capacity? what in Spain is called not falling into technocracies and being...

25 May 2020 4,481 18 View

Which are the different immunity mechanisms our organisms possess in order to protect us from lung cancer?

I was wondering if our immunological system possessed any kind of immunological defense to prevent lung cancer and how it deferred (if any) from any other kind of immunological reaction.

30 March 2020 3,691 3 View

Is direct Corona action via AC2 R or general primary inflammatory response the culprit?

In response to viral infection via respiratory system a mucus defense associated with the primary inflammatory response 1 to 3 days after infection, to be followed by immune reactivity some two...

28 March 2020 5,350 4 View

How do we overcome the ethical dilemma of doing an auto-ethnographic research, especially what to tell and what to hide?

also how do we not be defensive while writing an autoethnography? please do share your thoughts or any papers which can guide me. thanks,MD

10 December 2019 6,222 3 View

Tools for measuring patient experience in real practice?

I am interested in different tools that have been implemented in real practice for measuring patient experience (not patient satisfaction), for instance in Spain a questionnaire called IEXPAC is...

02 December 2019 6,609 8 View