We are interested to do some KD of our favorite lncRNA with the Cas13d describing in the paper: Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors. Konermann S and al.
As anyone tried it ? Which tool are you used to design the guides ?
Thank you for your help,
Christian Braun
Hi Christophe, not sure if this tool works for ncRNAs, but it might be worth a try: https://chopchop.cbu.uib.no
Christian
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Karim Rahimi
No need to look for special PAM sequence, just select your target RNA, choose the region of targeting across the transcript and design a 30 bp gRNA spacer which should be a reverse complement to your RNA of interest.
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