I am confused by your question. Can you explain in more detail, what you want to accomplish?

Dear sir,

Usually we used to estimate gaba for glutamate decarboxylase enzyme assay, by using GABAse enzyme or through HPLC methods. However GABAse is too costly and HPLC technique is time consuming. Is there any other method like zymogram or agar plate assay to confirm the glutamate decarboxylase enzyme activity. I tried to do zymogram technique, but i was not successful. In case sir, if you have any method for easy characterization, which is comparatively cheaper and less time consuming. Sir, I hope i clarified my question. 

Regards

Sakthi kumaran

Senior research fellow

CSIR-CFTRI (INDIA)

Hi there,

There are colorimetric assays for GAD taking advantage of the proton consumption (increase of pH) using bromocresol green indicator. For more info:

http://www.sciencedirect.com/science/article/pii/S0141022911001244

Thank you very much sir, for the kind reply and for clearing my doubts..

Regards 

Sakthi kumaran

CSIR-CFTRI

INDIA