I am preparing niosomes from mix of Span 40,60,80 and Cholestrol and I want to use them for drug delivery. It has been long I am struggling with the concentration of ingredients. As I use 100mg for 10 ml of hydration medium which is equal to 2 e-4 mol/L, but the amount is very small to manage ( when weighting) .
My method is thin film hydration method and for now the molarity of Span which I prepare at first ( 50 cc solution of Chloroform/methanol) is 2 e-4 . I just wonder how I can determine the range of concentration which I will have the niosomes.
Thanks,
Use stock solution dilution (after dilution another dilution on the first diluted, mean multiple dilutions) and vesicle bleach or hydrolysis and UV (Spectrophotometric, concentration based, dilution curve estimation) or HPLC-based quantitations.
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