How come that the peaks in MALDI-TOF-MS (VITEK Biomerieux) vary in brightness? - FAQS.TIPS

Eli Jong @Eli_Jong

09 March 2021 1 7K Report

Hi everyone,

I've been working on the detectability of alpha-defensin in urine for some months now and there is one thing i cant quite wrap my head around. The variety in brightness of the peaks in the generated spectra. (What determines the intensity is clear to me)

An explanation for the variety in brightness of the peaks might be that the database recognizes certain peaks and therefore make them brighter in the generated spectrum though im not sure if this is the case.

My question:

How come that the peaks in MALDI-TOF-MS (VITEK Biomerieux) vary in brightness?

Thank you.

Hello,

Thank you for your response!

In the attached file you can find one of the many spectra. It shows some peaks which are a darker red than some of the other peaks in the spectrum.

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