For pure culture enrichment - you can try fluid thioglycollate broth for each. https://www.nelabservices.com/pdf/tech-sheets/fluid-thio-2016.pdf .

Candle jar does not establish an anaerobic environment.

Hi Sa'Adatu,

To make a liquid anaerobic, you need to degassing it (bubbling) with nitrogen gas. you can bubble the bottle containing the liquid before sterilization step. If you want to bubble the liquid after sterilization, you must use a 0.2 um sterile filter.

For preparing anaerobic atmosphere, you can use candle or specific kits.

Best wishes

Duried

Some of important steps you have to follow to prepare the liquid media suitable for anaerobes: 1) preparation of culture media, 2) purgation of oxygen from the media by bubbling with anoxic gas, 3) sterilization of the sealed vessels by autoclaving, and 4) addition of reducing agents