I have a plant extract in 2.5 to 10% Tween 80. I intend to analyze it via HPLC to quantify catechin. While literature often uses cloud point extraction to concentrate the surfactant-rich phase, I am afraid that this might damage the column. Does anyone have any experience in HPLC quantification of non-ionic surfactant-containing extract? I am also concerned about the interference of Tween 80 with the chromatogram reading.