How can we send questions to colleagues to invite them to answer with the new RG format?

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Hi all, My research questionnaire has 3 sections each having a mix of yes/no and multiple choice questions. How can I test its reliability ?

The sections are about awareness, practice, and perception regarding waste management. Thanks

20 February 2021 5,722 3 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

17 February 2021 384 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

How to assign two different material/section properties in Abaqus(UMAT)?

Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...

11 February 2021 9,607 3 View

Michael W. Marek

When you find the answer, please be cautions about using this capability.

I have had to unfollow people who flooded my inbox with daily requests to comment on topics that were not of interest to me.

This kind of invitation to comment should be done sparingly, rather than as a standard procedure for every question, in my opinion.

Michael Heine

Dear Brenda,

I agree with Michaels view. Answering a question seriously needs time and should be optional to the reader. Getting a flood of questions from the same person is also limited by the RG system. As far as I know there is a locking of your account for some time if you are adressing more than 20 RG members with the same question.

With best regards

Michael