Induce sporulation, culture transferred to Malt Extract Agar (MEA) or Oatmeal Agar (OA) or Potato Dextrose Agar (PDA) or V8 -juice Agar mediun amended with streptomycin sulphate (0.05 g/L), and chloramphenicol (0.05 g/L).and incubated for 3 wk at 25 °C under 12 h dark/12 h fluorescent light regime.
Yea, I second Dr. MD's response. One can also use any of the media, and incubate at 25°C for 7days, on the 7th day slightly scrape the mycellium and incubate under near UV light for 3days.
Use the (PDA) or V8 -juice Agar medium added with streptomycin 40 µg/ml .and incubate for 2 week at 25 °C under 12 h dark/12 h fluorescent light regime. In case the V8 juice you must adjust the PH 4.5
After two week, pore the 10 ml autocalved distilled water on the petri plate and scratch by the autoclaved slide. and then filtered the suspension by the 2 layers cheese cloths, thereby it would be checked by the microscope. If not abundance, then the filtered suspension would be kept in the clean bench for 3 days more by the 16 h light and 8 h dark condition.
that is enough for abundance conidia.
How can we produce abundant conidia of Magnaporthe oryzae pathotype triticum? - ResearchGate. Available from: https://www.researchgate.net/post/How_can_we_produce_abundant_conidia_of_Magnaporthe_oryzae_pathotype_triticum [accessed Jul 13, 2016].
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