Actually, I have run a UV to check binding of boronic acid with diols .(ARS and with glucose,fructose galactose and mannose). I have obtained a graph with change in absorption intensities. But I am unable to explain it. Any suggestions?
it looks like your absorbance is too high after you've changed the sample. it should be less than 3, better lying around the value 1. so a lower concentration or a shorter pathlength may help.