Can any one help me in giving the protocol that :
How can we evaluate the phytoaccumulation potential of Arsenic (III) and Chromium (VI) by Eichhornia from the hydroponic medium?
First you have to prepare treatments (various concentration of As(III) and Cr(III)) and also control (no heavy metals, only growth medium) and placed the Eichhornia on it. Collect the water samples at 0 hour (beginning) and collect again when you end your experiment. Measure As or Cr concentration. Now comes about plant. Measure As and Cr conc. in the plant parts too. Now you can can calculation Accumulation factor% = (Conc. in the plant parts/conc. in the medium)*100
And then you have to see some other journals and compare these information and find how far your plant good for removal of As or Cr from the medium.
you can also calculate Uptake efficiency from above data
Generally, metal accumulation trend in the plant is the following (attached illustration)
So, this is the very brief way, how to you find phytoaccumulation potential for your plant species on As and Cr
In addition to the excellent answer given above, I would like to add - don't forget a blank treatment and don't forget to add growth solution containing essential elements, certainly if you intend to go for more prolonged growing experiments.
Thanks Erik, I have missed to write the control treatment....but its unintentional
Take into account that doing a routine analysis of Cr concentrations in the plant tissue you do not distinguish whether accumulation is in the form of Cr(VI) or Cr(III) you only can ascertain accumulation of the element, but not the species.