How can we determine Fe(II)/ Fe(III) in a porphyrin ring?

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Reduction of H2O2 at Glassy carbon electrode using Cyclic voltammetry?

I am trying to reduce H2O2 at GCE and other modified electrodes using cyclic voltammetry. But I am not getting well defined reduction peak. Can anybody tell what could be the reason?

09 October 2016 5,198 5 View

In pH-meter does the glass electrode acts as anode or cathode?

If it is acting as cathode then why?

07 August 2014 3,288 3 View

Which is best in CPE and GCE for film depositon and why?

Why is CPE used instead of a glassy carbon electrode, which is more common in film deposition?

06 July 2012 7,763 2 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

My article: The Extended Pedersen hypothesis can you correct the the name of first Author?

In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...

11 July 2024 1,801 2 View

Is it possible to generate a (semi-)permanent "transcription bubble" within a replicable plasmid?

I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...

07 July 2024 8,275 5 View

How to dilute essential oil from plants to assess the biological activity?

I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...

04 July 2024 8,100 4 View

Nutrition map: What does it mean for you?

Unluckily, just similar to an expensive car, your brain can be damaged if you ingest anything other than premium fuel.

23 June 2024 2,287 7 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

Luciferase assay lysis?

Hi! I'm doing a renilla luciferase assay with coelenterazine and HEK293T cells transfected with AP-1 renilla luciferase. I'm wondering if the cells need to be lysed prior to adding coelenterazine...

13 June 2024 9,936 5 View

How to prepare 0.28 U/ml working solution of Acetylcholinesterase inhibition assay for anti-Alzheimer activity?

I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...

31 May 2024 6,444 1 View

What is the difference between absorption and adsorption?

30 May 2024 4,447 27 View

What effect may biochar types produced from grape stems, leaves, onion leaves and stems, asparragus and cantaloupe stems/leaves have on a harvest?

I'm interested in knowing the differences in the effects caused by four different types of biochar: cantaloupe leaves/stems, onion leaves/stems, grape peels/stems/leaves and asparragus...

28 May 2024 4,907 0 View

Richard A Decréau

look up the UV/Vis of the Soret Band; the shift is significant. It also works with the Q band, but they are not as intense as the Soret (you also have to know if your system 5-coordinate or 6-coordinate)

Antonio Rosato

Richard is right. Alternatively, use EPR - does not require to know coordination