We use simple UV lamp (UV-C) to irradiate an insect. Is there any other source we can use? How to control the radiation dose? Please mention the source of UV-B.
Dear Sandip,
I fear that calculations of UV dose or irradiance from scratch are not easily done. I havn't heard from such calculations at all.
The simplest way for you is to use calibrated UV power meters. They are commercially available for UV-A, -B, -C.
With respect to your UV-B source question:
please use an appropriate UV light source ( e.g. Hg lamp) in combination with an UV-B filter (such filters are also commercially available). For UV-C and -A you can proceed also this way.
1) To determine accurately the UV dose received by a target, you need to determine
a) the spectral emission of your UV source by mean of an calibrated spectro-radiometer.
b) to define a "reference" distance between your UV source and your target. In a first approximation, intensity is proportional to the inverse of the square of the distance.
c) to apply the "action spectrum" to the absolute emission of your source for a define distance.
2) UV sources are various and available commercially
for instance UVC can be generated by Deuterium lamps (usually 40 W), UVB and UVA can be generated by tungsten ribbon source (100w, 200W, 500W or 1KW)
All these sources require stabilized power supply...
Hope that these few remarks can help you
Didier
Just a comment to complete my previous answer...
Be careful with Hg lamps or hollow cathode sources... all these UV sources emit single wavelengths and are not very appropriate to irradiate a biological target.
Didier
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