I want to estimate phenol in methanol crude extract and I dissolve 1 mg/ml of crude extract as a sample then i took 500 μL of it then add 100 μL of FC (50%) reagent then add 500 μ of 20% Na2CO3 then incubate it for one h then i took 200 μL of it to 96 plat then i read absorbance at 750 nm ( Same i did for gilic acid i prepared in deffrent conc ( 5, 10, 15, 20, 25 ,30) μg/ml and i add DW to make volume 500μL then i add 100μL FC then 500μL of NaCO3 then incubation for 1 h then i took 200μ of every conc to 96 plate and i read absorbance How can we calculate the total phenols in the methanol extract ?
Use your Gallic acid standard curve (y = mx + c )to calculate the total phenolics in your samples.
Well as I can see from the standard curve you shared
y=0.0472x
Here 'y' is the absorbance value on your Y axis and 'x' is your concentration (ug/ml) on the X axis
Suppose your sample has an absorbance of 0.1
So your equation will be:
0.1=0.0472x
Therefore, x=0.1/0.0472
x=2.11 ug/ml (this will be your phenolic content in ug/ml)
Hope it is helpful for you.
Niti Sharma Thank you so much ma'am
I got it ma'am and i got by this method same what i got from form this excel f (=TREND(G158:G163,H158:H163,E170)) which is 20.3 ug/ml But ma'am How can i got TheTotal Phenolic Content TPC mg/g.
The conc of crude extract was 1mg/ml and the volume of it i took 0.5ml
- Badges
- Science topic