What are the different methods to evaluate circadian rhythm in rodents (rats and mice)? One method I found is Actimetrics which is not available in our lab. Can anybody suggest any alternative method
Motion sensor logging might be useful.
An automatic open-field ambulation apparatus can register circadian rhythm in rodents:
Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...
30 July 2024 5,123 4 View
final solution volume is 100ml
03 June 2024 417 0 View
could you please help with a protocol? should i do it directly on the aluminium stub after dehydration with 100% alcohol or not? should i release the pollen or whole anther is better and dehisce...
03 June 2024 4,793 8 View
SPK tool steels are used for cutting wires made from low carbon steels. We are searching for a cheap and effective method for increasing the cutting characteristics of the cutters.
14 May 2024 4,948 0 View
Hello I recently encountered a problem with the reciprocal of step size plot in Comsol Multiphysics. After a certain time (seconds) in a time-dependent solver, the plot oscillations will be...
04 May 2024 7,278 0 View
Hi everyone I produced a batch of virus , enriched it with centrifugation, and titrated it with T cells after 48h. For my experiments I want to use MOI 3 and that's equal to 1/12 dilution. but at...
02 May 2024 9,727 3 View
InfoScience Trends offers comprehensive scientific content that delves into various facets of information science research. This includes but is not limited to topics such as information...
26 April 2024 9,521 1 View
Garret, 2020 used traps of blue, yellow, and white colors for sampling and collected several specimens.
06 April 2024 6,995 2 View
What are the laboratory procedures for identifying the concentrations of formic acid and acetic acid in aqueous solutions using titration, spectrophotometry, or gas chromatography methods...
06 April 2024 187 3 View
Is it possible to produce a very small amount of volatile oil along with condensed gas? If so, could you please provide a real example? Thank you.
04 April 2024 1,411 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View