How can the pH of a plant extract or fraction (dried) that is not soluble in water but only in organic solvent be determined. Considering the fact that when dessolved in an organic solvent, the pH could be affected.
pH of a solid cannot be determined nor pH is defined for pure organic solvents e.g. benzene. However you can measure pH of a suspension in water (at least in principle). What functional groups do you have on the molecule? What type of solvents dissolve it?
Hi what is the purpose of measuring pH of your plant extract ? Are you looking for organic acid or organic base present in your extract ?
pH measurement for solid usually done by dissolve / submerge solid samples into water, in a percentage of 10% - 50% (w/v) solid in water.
One of the standard method EPA 9045D was attached for your reference.
Hope this helps your research.
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