I have isolated more than 300 bacteria from rhizosphere, roots epiphytes and endophytes. Now which test should i conduct on bacterial isolate which can help to improve drought tolerance of the plant.
Please have a look at enclosed PDFs, quite interesting...
By making some physiological changes in terms of the biological metabolism and secretion of certain hormones they will increase the tolerance of cells to stress especially the ROS group
A possible mechanism is lowering plant growth rate. The same that is used in drought resistant GMOs. When plant is growing slower it need less water and survives better in drought conditions.
Now for sure you have to test these 300 strains for drought resistance and select the most drought resistant ones, especially those that maintain their usefulness (for example N fixation, etc) under drought conditions. The next step is greenhouse, and then field investigations with the crop of your choice.
Good papers presented here. Thank you all.
Thank you very much for your kind Answers, Your opinions will really help my current project.
Dear colleague Abid, please find hereby enclosed two articles related to the search of drought tolerant rhizobacteria. Both written in English and Spanish. Hope this could help you in some extent. Regards, Carlos
I suggest to perform ACC-deaminase activity of the isolated strains (either by biochemical method or by molecular anal). It is well established in literature that those strains which have ACC-deaminase activits, can help plants to mitigate stress (drought ) tolerance.
See our Review Article for more detail:
https://link.springer.com/article/10.1007/s13213-010-0117-1
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