My research lab has to maintain large quantity of potato germplasm. But I have to subculture for several times. How can I maintain those for long time conservation in a low cost ??
It is now accepted terminology to describe in vitro maintenance as 'medium term'. Long-term maintenance is through cryopreservation. There are many approaches to extend the subculture period, such as low light, low sucrose, low temperature - anything that slows down the in vitro growth. Potato is not difficult in cryopreservation and CIP (Dr Ana Panta) can help set up your facilities.
@ I think it can be stored easily for a long time. The farmers used to store their potato produce for months in a cold storage meant for this purpose. Similar method can be adopted if the quantity is too large. I think it is cost effective also.
Two things are suggested for your question :
1. Slow growth of cultures/ germplasm
2. cryopreservation.
Dear Dr. Hossain
Please following these cryopreservation studies
Article A large-scale viability assessment of the potato cryobank at...
Article Modified light spectral conditions prior to cryopreservation...
Good luck
Dear Altaf
Cryopreservation is becoming a very important tool for the long-term storage of plant genetic resources and efficient cryopreservation protocols have been developed for a large number of plant species. Practical procedures, developed using in vitro tissue culture, can be a simple and reliable preservation option of potato genetic resources rather than maintaining by vegetative propagation in genebanks due their allogamous nature.
You can maintain the cultures for medium term storage (6 months to one year) using sorbitoll and mannitol solution. Pls try to find out some protocols in the online journals.
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