I am using butanol fraction for isolation of compounds. But I got pink color spot also. are these plasticizer? if yes?? how can i remove them. i am using chloroform/methanol solvent system.
This pink color came from your sample. What compound are you trying to isolate/chromatograph and what is your sample matrix?
Dear Bruce Neagle,
My sample has plant extract (butanol fraction) and silica which is used for column chromatography.
Why do you feel there is plasticizer?
In any case, if you are sure the "pink spot" is a plasticizer, it will be removed as you run the column.
during isolation of compounds from plants some compounds appeared at 254 nm UV light and some at 365 nm. those which appeared at 365 nm what are they?? are they are molecule which can b drug?
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