tell me abou the pre-inocular preparation and the broth to use in liquid production and the grains to use for solid production to have more spores.
Grow it in Potato Dextrose broth and harvest spores, give a gentle blend and dilute it to 10,000000 CFU. This is good for inoculation. For mass production, inoculate sterilized bran/grains, incubate for 14 days, mix well the contents and get shade dry.
It can be grown even on various food wastes like wheat bran. Lots of literature available on this aspect.
thanks for the responses....i will try them out and see the results
T. harzianum can be mass multiplied on millet or other grain. Grains colonized by Trichoderma were air dried in open shade and ground with the help of Willy Mill to get fine powder. This powder should be passed through 50 and 80 mesh sieves simultaneously to obtain spore powder. Spore powder may be diluted with talcum powder and 1% carboxyl methyl cellulose (CMC) to get desired concentration (106cfu/g) of Trichoderma in the formulation.
- Badges
- Science topic
- Similar topics
- Geoscience
- Atmospheric Sciences
- Climate Science
- Climate Change