Hi guys,

i am just curious are there any methods or assays (bioinfo tools) to access protein internal rotation coming from single amino acid. My protein has a mutation, replacement of Glycine to arginine, as glycine is significantly small than highly basic and large side chained arginine. i am wondering how gross that effect could be incase of steric hinderance, lateral bonds formation and free internal rotation.

another important thing glycine was present in middle of two lysine's (KGK) so now (KRK) can also be effected due to surrounding basic lysin's.

any clues please write

Thank you.